Endothelin Peptide Protocols.- Immunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting Enzymes.- Analysis of Endothelins by Enzyme-Linked Immunosorbent Assay and Radioimmunoassay.- Separation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by Radioimmunoassay.- Endothelin Receptor Protocols.- Radioligand binding assays and quantitative autoradiography of endothelin receptors.- Using Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin Receptor.- Quantification of Endothelin Receptor mRNA by Competitive RT-PCR.- Endothelin-Converting Enzyme Protocols.- Detection and Assessment of Endothelin-Converting Enzyme Activity.- Quantitative Measurement of mRNA Levels by RT-PCR.- Evaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells.- Endothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In Vitro.- In Vitro Functional Assays.- Characterization of a New Endothelin Receptor Ligand by In Vitro Assays.- Use of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal Proliferation.- In Vivo Functional Assays.- Investigation of the Endothelin System in Experimental Heart Failure.- Local Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In Vivo.

"This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..." -Acta Cardiologica